ENGINEERING HDR SEMINAR SERIES (44/2013) : Development of bioinformatics approach for soluble periplasmic expression in Escherichia coli

Ms Catherine Chang Ching, postgraduate student from School of Engineering, Monash University Sunway Campus

Date: 2013-09-26
Time: 11:00 to 12:00
Venue: Engineering Meeting Room 1, 5-4-22


Escherichia coli is known as the most widely used expression system for the production of various non-glycosylated recombinant proteins. Numerous strategies have been developed to enhance the production yield of recombinant protein in E. coli. However, all these strategies involve series of trial and error steps with unguaranteed success. In addition, recombinant proteins are frequently overexpressed in the form of inclusion bodies in cytoplasm, which subsequently necessitates complex separation, denaturation and refolding processes. These are inevitable yet time-consuming, costly and complicated processes. In this research work, a systematic approach was developed to predict the amount of soluble protein expressed in the periplasm of E. coli utilizing the available bioinformatics tools. By utilizing the outcome from this research work, the trial and error procedures involving in vivo experimental work can be avoided. Subsequently, this will possibly allow rational target selection of the most promising candidates of signal peptide – target gene combination prior to in vivo experimental work.

About the Speaker

Catherine Chang Ching Han graduated with a Bachelor of Engineering (Chemical Engineering with Honours) from Monash University Malaysia in 2012. She joined Monash University in the same year for postgraduate studies under the supervision of Dr. Ramakrishnan Nagasundara Ramanan and Prof. Tey Beng Ti. Her research focus is on bioinformatics approaches for improved soluble recombinant protein production in the periplasm of Escherichia coli.