Seminar

ENGINEERING HDR SEMINAR SERIES (48/2013) : Electrical Response of Protein Antibody Interaction in Surface Acoustic Wave Based Biosensors

Mr Chu Yih Bing, postgraduate student from School of Engineering, Monash University Sunway Campus


Date: 2013-10-29
Time: 10:00 to 11:00
Venue: Meeting Room 2-4-30


Abstract

SAW devices are well known for their use in signal processing circuits, sensors, and actuators. Sensors based on SAW usually consist of a sensing medium made of thin film coated over the SAW path and changes in mass loading, conductivity, viscosity and permittivity of film caused by a measurand will introduce changes in velocity, amplitude and phase of the SAW. These changes can be measured with simple oscillator circuit as shift in resonance frequency and alteration in gain. In the present study we proposed to work on devising a SAW based biosensor consisting of immobilized monoclonal anti-α-Interferon Mouse mAb (MMHA-2) antibody adsorption on CMC-cellulose film to detect human Interferon-a2b (hIFN-α2b) protein and investigate the electrical response for presence of specific concentration of protein. We propose to model the protein-antibody interaction with SAW inform of electrical equivalent circuit based on precise measurement of mass loading and electrical parameters through experiments. The research work involves devising SAW devices, optimizing immobilization protocol on the surface of SAW device, integrate microfluidic setup with appropriate micropump assemblies, and interface the sensor with microprocessor based measurement circuit. 

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About the Speaker

Mr Chu Yih Bing graduated with Bachelor of Mechatronics (Hons) from Monash University Malaysia. He  continued his postgraduate studies studies under the supervision of Dr. Narayanan Ramakrishnan, Dr. Ramakrishnan Nagasundara Ramanan and Dr. Tuncay Alan. His study focuses on to model the protein-antibody interaction with integrated Surface Acoustic Wave (SAW) based biosens or inform of electrical equivalent circuit through precise measurement of mass loading and investigation of electrical parameters from experiments.